Table 4 The gram-scale kinetic resolution of rac-3a using 200 mg wet cells/mL of E. coli/pveh3E134K/T137P.

From: Significant improvement in catalytic activity and enantioselectivity of a Phaseolus vulgaris epoxide hydrolase, PvEH3, towards ortho-cresyl glycidyl ether based on the semi-rational design

Rac-3a conc. (mM)

Time (h)

c (%)

ees (%)

Yield (%)

200

1.8

59.7 ± 1.7

99.7

40.2 ± 1.3

400

4.0

59.9 ± 1.7

99.6

39.0 ± 1.2

600

5.0

60.8 ± 1.8

99.1

39.1 ± 1.1

800

7.0

61.4 ± 1.6

99.4

38.5 ± 1.2

1000

14.0

44.2 ± 1.3

74.8 ± 2.1

−

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