Figure 3 | Scientific Reports

Figure 3

From: CAPA neuropeptides and their receptor form an anti-diuretic hormone signaling system in the human disease vector, Aedes aegypti

Figure 3

Mapping of anti-diuretic hormone in the abdominal ganglia of the central nervous system and associated neurohaemal organs in adult A. aegypti. (A) Immunohistochemical distribution of CAPA neuropeptides in the abdominal ganglia (AG); specifically, a pair of highly immunoreactive neurosecretory cells within AG1–2 (A), AG3–4 (A’) and AG4–6 (A”). Higher magnification of AG3 (B) and AG4 (C) demonstrating CAPA immunoreactivity within large ventrally-positioned neurosecretory cells with axonal projections emanating dorsally within the ganglia and projecting anteriorly into the median nerve. (D) CAPA immunoreactivity in abdominal preparations with dorsal cuticle removed leaving the ventrally-localized AG within the abdominal segment showing immunoreactive processes innervating the abdominal neurohaemal (perivisceral) organs. CAPA transcript localization by fluorescent in situ hybridization revealing pairs of neurons within each AG including AG1 (E), AG2 (E’), AG3–4 (E”) and AG5 and the terminal abdominal ganglion (TAG; E”’). Co-localization of CAPA immunoreactivity (F) and CAPA transcript (G) was verified in all abdominal ganglia with representative preparation in (H) showing transcript and immunoreactivity co-detection and overlap. Scale bars: 200 µm (A,D), 100 µm (B,C,E) and 50 µm (F,H).

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