Figure 4 | Scientific Reports

Figure 4

From: CAPA neuropeptides and their receptor form an anti-diuretic hormone signaling system in the human disease vector, Aedes aegypti

Figure 4

RNA interference (RNAi) of CAPAr abolishes anti-diuretic activity of CAPA neuropeptide on adult female A. aegypti MTs. (A) Verification of significant knockdown (>75%) of CAPAr transcript in MTs of four-day old adult female A. aegypti by RNAi achieved through injection of dsCAPAr on day one post-eclosion. (B) Functional consequences of CAPAr knockdown demonstrating loss of anti-diuretic hormone activity by AedaeCAPA-1 against DromeDH31-stimulated fluid secretion by MTs. In (A), knockdown of CAPAr transcript was analyzed by one-tailed t-test (* denotes significant knockdown, p < 0.01). In (B), fluid secretion rates by MTs presented as mean ± SEM and analyzed by one-way ANOVA and Tukey’s multiple comparison post-test, where different letters denote treatments that are significantly different (p < 0.05, n = 14–33).

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