Table 6 List of candidate reference genes and details of primes used for stability analysis.
Gene symbol | Gene Name | Accession number | Sequence (5′->3′) forward and reverse | Amplicon length (bp) | Ta (ºC) |
|---|---|---|---|---|---|
ACT1 | β-actin | XM_002549283.1 | CGTCGGTAGACCAAGACACC CCCAGTTGGAGACAATACCGT | 137 | 59 |
EF1 | Elongation factor 1α | XM_002547480.1 | GGTCAAACCAGAGAACACGC TTCTTCAAATCTGTTTTTGTCCCA | 111 | 59 |
GAPDH | Glyceraldehyde 3-phosphate dehydrogenase | XM_002551322.1 | TTACGAAGAAATTTGTGCTGCT AGCATCAAAGACAGAGGAGTAAGA | 130 | 59 |
PGK1 | Phosphoglycerate kinase | XM_002548594.1 | GCTGACGCTGTCGGTAAAG GCAGAAGCAACACAGGCA | 116 | 59 |
RDN5.8 | 5.8S ribosomal RNA | AB437083.1 | GAGCAATCCTACCGCCAGAG TGCGAGAACCAAGAGATCCG | 113 | 59 |
RDN18 | 18S ribosomal RNA | M55527.1 | GTGCTGGCGATGGTTCATTC CGTTTCTCAGGCTCCCTCTC | 125 | 59 |
RDN28 | 28S ribosomal RNA | KY106836.1 | GTGAAGCGGCAAAAGCTCAA CACCCTCTGTGACGTTCTGT | 123 | 59 |
SDHA | Succinate dehydrogenase complex | XM_002549452.1 | TTCGTAACCAAATAAGAAGTTCCGC GCTCATGTATTTGGCAGCGTTA | 119 | 59 |
TUB1 | α-tubulin | XM_002546417.1 | TTGACTGGTGTCCAACTGGT CAGCAATAGCGGTAGTGTTAGA | 126 | 59 |
UBC13 | Ubiquitin-conjugating enzyme E2 13 | XM_002550926.1 | AGTATTCAAGCTTTGTTAGGTGCTC GAGTTTAGTCCATTCTTGAGCCAT | 120 | 59 |
