Table 1 Demethylation of methylcob(III)alamin (MeCbl(III)) catalyzed by core-MetECBDB from D. mccartyi strain CBDB1 in the presence of D,L-homocysteine at different pH values.
From: Identification and characterization of a bacterial core methionine synthase
pH | MeCbl(III) consumption [µM min−1] | relative activity [%] |
|---|---|---|
5.0 | 20.53 ± 4.46 | 82.0 |
5.5 | 19.12 ± 3.23 | 76.4 |
6.0 | 25.05 ± 0.37 | 100 |
6.5 | 23.87 ± 1.41 | 95.3 |
7.0 | 18.96 ± 0.07 | 75.6 |
7.5 | 21.61 ± 0.41 | 86.3 |
8.0 | 18.08 ± 1.02 | 72.3 |
8.5 | 17.70 ± 0.88 | 70.6 |
9.0 | 14.23 ± 0.61 | 56.9 |
