Figure 4 | Scientific Reports

Figure 4

From: Discovery of a novel dehydratase of the fatty acid synthase type II critical for ketomycolic acid biosynthesis and virulence of Mycobacterium tuberculosis

Figure 4

HadDMtb protein is implicated in keto-mycolic acid biosynthesis pathway. (A) Structures of the main MA classes produced by M. tuberculosis. One of the main molecules was drawn for each class, as an example. The three segments of the meromycolic chain, delimited by the distal (D) and the proximal (P) positions of the chemical functions, are indicated. (B) Mycolic acid methyl ester (MAME) profiles in HPTLC. Five µg of MAMEs from each strain were loaded onto a HPTLC plate developed five times in petroleum ether/diethyl ether, 9:1 (v/v) and stained by immersion in CuSO4 and heating. The figure is representative of six independent experiments. The hydroxy-MAs are minority MAs bearing an hydroxyl group at the distal position, and precursors of the keto- and methoxy-MAs28. (C) MA distribution in the different strains deduced from the quantification of the HPTLC band intensities as in panel B. Data are means ± average deviations of at least six independent experiments.

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