Figure 1 | Scientific Reports

Figure 1

From: Genome-wide screening of lipoproteins in Actinobacillus pleuropneumoniae identifies three antigens that confer protection against virulent challenge

Figure 1

Purification of recombinant lipoproteins expressed in the supernatant of E. coli lysate. Recombinant proteins were purified from E. coli lysate using a glutathione–Sepharose 4B affinity chromatography column, separated by 12% SDS-PAGE and stained with Coomassie Brilliant Blue. Lane M, prestained protein ladder (Fermentas, Vilnius, Lithuania); lane C, recombinant GST control; other lanes: recombinant lipoproteins, the lane number indicates the protein number of A. pleuropneumoniae JL03.

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