Table 1 Bacterial strains and plasmids used in this study.
Strains, plasmids and primers | Relevant characteristics | Sources |
|---|---|---|
A. pleuropneumoniae | ||
JL03 | serovar 3 | Field isolate |
4074 | serovar 1 | From Dr. Pat Blackall |
E. coli | ||
DH5a | Cloning vehicle: supE44 △lacU169 (φ80 lacZ△M15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Takara |
BL21(DE3) | Expression host: F− ompT r−B m−B; DE3 is a λ derivative carrying lacI and T7 RNA polymerase genes under placUV5 control | Takara |
Plasmids | ||
pMD18-T | E. coli cloning vector carrying an ampicillin resistance determinant. | Takara |
pMD-APJL_ | pMD18-T carrying the coding sequence for lipoprotein of A. pleuropneumoniae JL03, for sequence analysis. | This work |
pGEX-KG | N-terminal glutathione S-transferase (GST) fusion expression vector: pBR322 ori, Amp r. | Ref. 44 |
pGEX-APJL_ | pGEX-KG carrying the coding sequence for lipoprotein of A. pleuropneumoniae JL03, for the expression of GST-fusion recombinant protein | This work |
