Table 3 IgG titers of pigs vaccinated with different immunogens.

From: Genome-wide screening of lipoproteins in Actinobacillus pleuropneumoniae identifies three antigens that confer protection against virulent challenge

Group

Immunogen

IgG titer against APJL_0922 (mean ± SD)a

IgG titer against APJL_1380 (mean ± SD)

IgG titer against APJL_1976 (mean ± SD)

Day 14b

Day 28

Day 14

Day 28

Day 14

Day 28

1

APJL_0922

2.75 ± 0.13 c,d

3.97 ± 0.16c,d

ND

ND

ND

ND

2

APJL_1380

ND

ND

2.69 ± 0.16c,d

4.17 ± 0.25c,d

ND

ND

3

APJL_1976

ND

ND

ND

ND

2.69 ± 0.50d,e

3.75 ± 0.39c,d

4

Bacterin

1.78 ± 0.16d

3.32 ± 0.16d

1.66 ± 0.13  f

2.48 ± 0.16d

1.72 ± 0.16  f

2.36 ± 0.13d

5

Negative control

1.18 ± 0.16

1.38 ± 0.25

1.30 ± 0.21

1.16 ± 0.22

1.36 ± 0.25

1.32 ± 0.18

  1. aThe solid-phase antigen in ELISAs were prepared as described in the text. The results given are the arithmetic mean log10 values of the reciprocal of the highest serum dilution showing an optical density twice as high as the negative control serum.
  2. bDay 14 means before the second vaccination; Day 28 means before challenge.
  3. Differences in antibodies titers among groups were calculated by one-way ANOVA followed up with Student’s t-test to compare between any 2 groups. All ANOVA tests were found to be significant (P < 0.001 for ELISA titers against APJL_0922 and APJL_1380; P < 0.01 for ELISA titers against APJL_1976).
  4. For Student’s t-test between two groups, cP < 0.001 compared to bacterin, dP < 0.001 relative to negative control, esignificantly different from bacterin (P < 0.01), fsignificantly different from negative control (P < 0.05).
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