Table 2 Summary of STR-based and microhaplotype-based analysis of artificially mixed biological samples.

From: A 124-plex Microhaplotype Panel Based on Next-generation Sequencing Developed for Forensic Applications

Genetic marker

Mixtures

Number of loci with fully called 9947 A alleles

9947 A drop-out loci

Number of loci interfered by stutters in mixture deconvolution

Loci interfered by stutters in mixture deconvolution

Number of remaining effective loci

Percentage of remaining effective loci

STR

9947 A:2800 M = 1:1

21

 

0

 

21

100.00%

9947 A:2800 M = 1:3

21

 

0

 

21

100.00%

9947 A:2800 M = 1:6

21

 

7

D16S539, CSF1PO, D18S51, D19S433, FGA, D22S1045, D2S1338

14

66.67%

9947 A:2800 M = 1:9

19

D22S1045, D2S1338

11

D3S1358, vWA, D16S539, CSF1PO, D18S51, D19S433, FGA, D5S818, D7S820, D10S1248, D12S391

8

38.10%

9947 A:2800 M = 1:19

8

D3S1358, vWA, D16S539, CSF1PO, TPOX, D18S51, D19S433, TH01, D22S1045, SE33, D1S1656, D12S391, D2S1338

7

D8S1179, D21S11, FGA, D5S818, D13S317, D7S820, D10S1248

1

4.76%

Microhaplotype

9947 A:2800 M = 1:1

124

 

0

 

124

100.00%

9947 A:2800 M = 1:3

123

mh02KK-136

0

 

123

99.19%

9947 A:2800 M = 1:6

123

mh02KK-136

0

 

123

99.19%

9947 A:2800 M = 1:9

123

mh02KK-136

0

 

123

99.19%

9947 A:2800 M = 1:19

114

mh01KK-205, mh02KK-136, mh04KK-019, mh05KK-079, mh07KK-030, mh08KK-032, mh10KK-087, mh12KK-043, mh17KK-055, mh21KK-324

0

 

114

91.94%