Figure 1
Phylogenetic tree indicates SRLV subtypes and their differing geographical distribution (country). The analysis was performed using the Maximum Likelihood method and was based on the Tamura-Nei model61. The analysis involved a total of 399 bp from 65 nucleotide sequences: 17 German gag sequences (labelled by solid red circles), six Iranian sequences (labelled by solid blue circles), two Jordanian SRLV sequences (KT898826 and KT921318), a Lebanese SRLV sequence (KU170760) and 39 reference SRLV sequences originating from different geographical areas (retrieved from the GenBank database). The position of the analysed fragments was related to coding regions of the capsid gene (p25) and the nucleocapsid gene (p14) located on the gag fragment (nucleotide: 1114–1506; numbering according to prototype strain K151462. The difference in the evolutionary rate among sites was considered using the discrete gamma distribution ( + G parameter = 0.5202) and the invariable sites ( + I = 21.68% sites). The numbers on the nodes indicate the percentage of bootstrap values obtained from 1,000 replicates. Bootstrap values of less than 60% were excluded from nodes. The branch lengths show the number of substitutions per site. Evolutionary analyses were performed in MEGA760. The two suggestive clades of genotype A, “European/Turkish clade” and “Middle Eastern/Iranian clade” are shown with arrows.
