Figure 3
Agrobacterium-mediated transient expression of Ppal15kDa in N. benthamiana. Total proteins were extracted from infiltrated N. benthamiana leaves and subjected to SDS-PAGE followed by Western blot with HRP conjugated anti-His monoclonal antibody. Lane M represents the protein standard and lane GFP represents N. benthamiana leaves infiltrated with A. tumefaciens GV3101 carrying pJL-TRBO-G. Lane A and B represent N. benthamiana leaves infiltrated with A. tumefaciens GV3101 carrying pJL-TRBO-Ppal15kDaA and pJL-TRBO-Ppal15kDaB, respectively. The arrows indicate two forms of Ppal15kDa. The full-length gel is presented in Supplementary Fig. 3 (Fig. S3).
