Figure 2
From: TALEN-based editing of TFIIAy5 changes rice response to Xanthomonas oryzae pv. Oryzae
The lesions length of rice leaves in the knock-out mutants 14 days after inoculation with PXO86. (a) The T2 plants and their derivative T3 plants were inoculated by PXO86 in Beijing in August, 2015 and 2016 respectively. (b,c) The T3 plants in MH86 or D62B background were examined in Hainan in February, 2016. (d) The disease symptoms of the T3 plants were photographed 14 days after inoculation. (e) The targeted sequences of TALEN-Xa5 in transgenic plants. WT is the initial target sequence of Xa5, and the others are the mutant sequences. TP309, MH86, and D62B. TP309, MH86, and D62B are blank controls; TP, 1TP, 2TP and 3T were transgenic controls in TP309 background with pCAMBIA1300 vector; 1MH and 2MH are transgenic controls in MH86 background; 1D6 is the transgenic control in D62B background. All controls had the same target sequence as the WT. The other rice lines were knock-out mutants with their target sequences shown in e. TP, 119, 123, 278, 23 and 179 are the homozygous T2 mutant plants. 1TP, 2TP and 3TP are the random selected T3 plants derived from the TP; 119-1, 119-2 and 119-3 are the T3 plants from the 119; 123-1, 123-2, 123-3, and 123-4 are the T3 plants from the 123. The 278-1 and 278-2 are homozygous mutant T3 plants from the 278. 23-1 and 179-1 are homozygous mutant T3 plants from 23 and 179 respectively. Bars represent the average ± SD of three biological repeats. Different letters above columns indicate significant differences at P < 0.05 as determined by a one-way ANOVA followed by post hoc Tukey honest significant difference (HSD) analysis.
