Figure 5

Lesion length in rice leaves of in-frame mutants inoculated with Xoo. (a) The homozygous T₂ plants of in-frame mutants were inoculated by PXO86 in Beijing in August, 2015. TP309 and MH86 were the control plants containing the gene Xa5; TP is the T₂ transgenic control plant from TP309 with pCAMBIA1300 vector. 133 is the T₂ plant from TP309 with mutant Xa5 protein, in which two amino acids were replaced with six amino acids in the exon 1 (type 3 in the Table 1). The mutant Xa5 proteins in O23, O32 and 3 all have an amino acid deletion in the exon 1 (type 1 in Table 1). 217 expresses a mutant Xa5 protein with four amino acid-deletion in the exon 1 (type 5 in Table 1). 235 expresses the mutant Xa5 protein, in which five amino acids were replaced with nine amino acids in the exon 1 (type 6 in the Table 1). IRBB5 contains the homozygous resistant gene xa5, and functions as the resistant control here. (b) The homozygous T₃ plants of in-frame mutants from D62B and IR24 were inoculated with PXO86 in Hainan in February 2016. D62B and IR24 are the rice varieties with homozygous Xa5 gene. 1D6 and 1IR are the T₃ transgenic control plants with pCAMBIA1300 plasmid derived from D62B and IR24 respectively. 180-1 expresses a mutant Xa5 protein with three amino acid-deletion in the exon 1 (type 4 in Table 1). 260-1 expresses a mutant Xa5 protein with an amino acid insertion in the exon 1 (type 8 in Table 1). 38-1 and 38-2 are the T₂ plants derived from 38 that have a nine amino acid-deletion in the exon 1 of the Xa5 protein (type 2 in Table 1). Bars represent the average ± SD of three biological repeats. Different letters above columns indicate significant differences at P < 0.05 as determined by a one-way ANOVA followed by post hoc Tukey honest significant difference (HSD) analysis.