Table 1 The DNA and protein sequencesof the gene TFIIAγ5 in in-frame mutants edited by TALEN-Xa5.
From: TALEN-based editing of TFIIAy5 changes rice response to Xanthomonas oryzae pv. Oryzae
No. | Target sequence of TALEN-Xa5 | Indels |
|---|---|---|
Xa5 | GATGGTCTCCAGCGGCACCCTCAGCCCGGAGCTCGCCATTCAAGTTCTTGTCCAGTTT | — |
1 | GATGGTCTCCAGCGGCACCCTCAGCCCG---CTCGCCATTCAAGTTCTTGTCCAGTTT | −3 |
2 | GATGGTCTCC---------------------------ATTCAAGTTCTTGTCCAGTTT | −27 |
3 | GATGGTCTCCAGCGGCACCCTCAGCCCGG---T----A---GAG--CTTGTCCAGTTT | −17/+5 |
4 | GATGGTCTCCAGCGGCACCCTCAGCCC---------CATTCAAGTTCTTGTCCAGTTT | −9 |
5 | GATGGTCTCCAGCGGCACC------------CTCGCCATTCAAGTTCTTGTCCAGTTT | −12 |
6 | GATGGTCTCCAGCGGCACCCTCAGCCCG-CGTGCGCC--T-AA--AC-T-T----TTT | −27/+15 |
7 | --------------------------------CTCGCCATTCAAGTTCTTGTCCAGTTT | −39 |
8 | GATGGTCTCCAGCGGCACCCTCAGCCCGGAG/CTCGCCATTCAAGTTCTTGTCCAGTTT | +3GAG |
xa5 | GATGGTCTCCAGCGGCACCCTCAGCCCGGAGCTCGCCATTCAAGTTCTTGAGCAGTTT | −2/+2 |
No. | First 50 amino acids of TALEN-Xa5 | changes |
Xa5 | MATFELYRRSTIGMCLTETLDEMVSSGTLSPELAIQVLVQFDKSMTEALE | — |
1 | MATFELYRRSTIGMCLTETLDEMVSSGTLSP-LAIQVLVQFDKSMTEALE | 32D |
2 | MATFELYRRSTIGMCLTETLDEMVS---------IQVLVQFDKSMTEALE | 26–34D |
3 | MATFELYRRSTIGMCLTETLDEMVSSGTLSP---VE---LVQFDKSMTEALE | 32–37D |
4 | MATFELYRRSTIGMCLTETLDEMVSSGTLSP---IQVLVQFDKSMTEALE | 32–34D |
5 | MATFELYRRSTIGMCLTETLDEMVSSGT----LAIQVLVQFDKSMTEALE | 29–32D |
6 | MATFELYRRSTIGMCLTETLDEMVSSGTLSP--RAPKL--FDKSMTEALE | 32–40R |
7 | MATFELYRRSTIGMCLTET-------------LAIQVLVQFDKSMTEALE | 20–32D |
8 | MATFELYRRSTIGMCLTETLDEMVSSGTLSPE/LAIQVLVQFDKSMTEALE | 32–33I |
xa5 | MATFELYRRSTIGMCLTETLDEMVSSGTLSPELAIQVLEQFDKSMTEALE | 39R |
