Figure 5

(A) TRIR spectra of dark- and light-adapted (blue and red respectively) W64F and the Y21F/Y56F/W64F AppA mutants (green) recorded at 10 ps; (B) Kinetics of the excited state of the flavin in the dark- and light-adapted (blue and red respectively) states of W64F AppABLUF and in the Y21F/Y56F/W64F AppABLUF (green) observed at 1380 cm-1.