Table 1 Fluorescence lifetimes (ns) of 7AW and corresponding amplitudes in the 7aza-W104/W64F mutant (no FAD present), and the 7aza-W104/W64Fmutant (FAD present) in the dark- and light-adapted states.
7azaTrp-W64F/W104 | τ1 | α1 | τ2 | α2 | τ3 | α3 | τaverage | reference |
|---|---|---|---|---|---|---|---|---|
apoprotein | 0.4 | 0.2 | 4.1 | 0.3 | 16.2 | 0.4 | 8.7 | this work |
holoprotein dark | 1.1 | 0.4 | 4.4 | 0.3 | 16 | 0.3 | 6.8 | this work |
holoprotein light | 1.3 | 0.4 | 3.5 | 0.3 | 14.7 | 0.2 | 5.2 | this work |
canonical Trp | 0.2–0.9 | 1.2–3.6 | 3.7–9.2 |
