Table 3 Fluorescence decay constants of the flavin and corresponding amplitudes in various AppABLUF mutants.

From: Functional dynamics of a single tryptophan residue in a BLUF protein revealed by fluorescence spectroscopy

Protein

A1

T1(ps)

A2

T2(ps)

R1 (Å)

R2 (Å)

W64F dark

0.7

500

0.3

70

9.5

8.03

W64F light

0.13

230

0.87

11

8.9

6.7

Y21F/Y56F/W64F

0.43

200

0.57

36

8.8

7.6

  1. Estimated distances between the flavin and W104 based on electron transfer calculations. The major contribution is shown in bold.
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