Figure 5 | Scientific Reports

Figure 5

From: A representative metalloprotease induces PGE2 synthesis in fibroblast-like synoviocytes via the NF-κB/COX-2 pathway with amplification by IL-1β and the EP4 receptor

Figure 5

NF-kB transcription factor is relevant to BaP1-induced COX-2 and mPGES-1 protein expression by FLSs. Isolated FLSs were pretreated with TPCK (25 μM) or SN50 (50 μg/mL) for 1 hour and stimulated by BaP1 (12.5 mg/mL) or RPMI (control) for 3 h. (A,C) Representative Western blotting of COX-2 and β-actin (loading control) showing immunoreactive bands. (B,D) Densitometric analysis of immunoreactive COX-2 bands. (E,G) Representative Western blotting of mPGES-1 and β-actin (loading control) showing immunoreactive bands. (F and H) Densitometric analysis of immunoreactive mPGES-1 bands. (E) Results are expressed as mean ± SEM of 3–4 samples. *p < 0.05 relative to control. #p < 0.05 relative to BaP1 (one-way ANOVA, Bonferroni post test). Blots were cropped from the full-length blots, which are demonstrated as Supplementary Figures 6(A–D) and 7(A–D) and cropped blots are indicated by arrows.

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