Figure 6 | Scientific Reports

Figure 6

From: A representative metalloprotease induces PGE2 synthesis in fibroblast-like synoviocytes via the NF-κB/COX-2 pathway with amplification by IL-1β and the EP4 receptor

Figure 6

Effect of BaP1 on NF-kB activity in FLSs. (A) Cultured FLS were exposed to 12.5 μg/mL BaP1 for 30 and 60 min. Nuclear extracts were then prepared and analyzed by electrophoretic mobility shift assay (EMSA). (B) Densitometric analysis of immunoreactive NF-kB bands. (C) Binding specificity to detected bands was confirmed by supershift assays with p50, RelB, p52, cRel and RelA. (D) IkB-α degradation. FLSs were exposed to 12.5 μg/mL BaP1 for 5, 15 and 30 min. Representative Western blotting of IkB-α and β-actin (loading control) showing immunoreactive bands. (E) Densitometric analysis of immunoreactive IkB-α bands. Densities (in arbitrary units) were normalized to β-actin densities. Results are expressed as mean ± SEM of 3–4 samples. *p < 0.05 relative to control (one-way ANOVA, Bonferroni post test in B and two-way ANOVA, Bonferroni post test in E). Blots were cropped from the full-length blots, which are demonstrated as Supplementary Figures 8(A–D) and 11(A–C) and cropped blots are indicated by arrows.

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