Figure 7
EP4 receptor antagonist blocks BaP1-induced effects in FLSs and BaP1up-regulates the expression of EP4 receptor. Isolated FLSs were pretreated with AH23848 (30 μM) and stimulated by BaP1 (12.5 mg/mL) or RPMI (control) for 3 hours. (A) Representative Western blotting of COX-2 and β-actin (loading control) showing immunoreactive bands. (B) Densitometric analysis of immunoreactive COX-2 bands. Densities (in arbitrary units) were normalized to β-actin densities. (C) Representative Western blotting of mPGES-1 and β-actin (loading control) showing immunoreactive bands. (D) Densitometric analysis of immunoreactive mPGES-1 bands. Densities (in arbitrary units) were normalized to β-actin densities. (E) PGE2 concentrations determined by ELISA. Results are expressed as mean ± SEM of 4 samples. (F) Representative Western Blotting of EP4 receptor and β-actin (loading control) showing immunoreactive bands. (G) Densitometric analysis of immunoreactive EP4 (54 kDa) bands. (H) Densitometric analysis of immunoreactive EP4 (62 kDa) bands. Densities (in arbitrary units) were normalized to β-actin densities. Data are mean ± SEM of 3 samples *p < 0.05 relative to control; #p < 0.05 relative to BaP1 (one-way ANOVA, Bonferroni post test in B, D and E and two-way ANOVA, Bonferroni post test in G and H). Blots were cropped from the full-length blots, which are demonstrated as Supplementary Figures 9(A–D) and 10(A–D) and cropped blots are indicated by arrows.
