Figure 3 | Scientific Reports

Figure 3

From: A recombinant avian paramyxovirus serotype 3 expressing the hemagglutinin protein protects chickens against H5N1 highly pathogenic avian influenza virus challenge

Figure 3

Multicycle growth kinetics and plaque morphology of rNDV or rAPMV-3 expressing HA protein in DF1 cells. The monolayers of DF1 cells were infected with rNDV, rNDV/HA, rAPMV-3 and rAPMV-3/HA at 0.1 MOI. The supernatant media containing virus were removed after one hour adsorbtion. The cells were washed and DMEM containing 10% allantoic fluid was added to cells. The titer of viruses in equal volumes of supernatants collected from infected cells in 8 hours intervals were detected by TCID50 using DF1 cells. (A,B) The plaque morphology of rNDV, rNDV/HA, rAPMV-3 and rAPMV-3/HA in presence of 10% allantoic fluid were evaluated in DF1 monolayers (C).

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