Figure 2
From: Chronic irradiation of human cells reduces histone levels and deregulates gene expression
Chronic γ-radiation reduces histone levels. (a) Western blot analyses of histone H2AX and γH2AX in primary fibroblasts exposed to various dose-rates of chronic γ-radiation for 7 days. Cells irradiated with a single acute dose of 4 Gy X-ray were included as control. (b) Effect of chronic γ-irradiation on H2AX levels in three different isogenic primary cell types from a different donor to that used in (a) at the same dose rates as in (a). (c) Immunoblots of other histones in chronically irradiated primary fibroblasts. (d) All significant histone level changes detected by SILAC LC-MS/MS protein analyses of samples from primary fibroblasts exposed or mock-exposed to chronic γ-radiation for 7 days.
