Figure 5
From: Chronic irradiation of human cells reduces histone levels and deregulates gene expression
Reduction of histone levels in chronically irradiated cells occurs mainly though reduced transcription, not protein degradation. (a) Effect of inhibiting the protein degradation capability in fibroblasts exposed to chronic radiation for 7 days. Proteasome inhibition (5 µM MG132 for 24 h) or lysosomal autophagy inhibition (10 µM chloroquine for 24 h) of fibroblasts exposed to chronic γ-radiation for 7 days. (b) Differential expression of histone gene transcripts from RNA-seq analyses of primary fibroblasts after 7 days exposure to 20 mGy/h chronic γ-radiation with changes greater than 1.5-fold and p < 0.01. (c) Changes in core histone levels in fibroblasts as measured by qRT-PCR after 7 days exposure to the same radiation conditions as in (b).
