Figure 6
From: Chronic irradiation of human cells reduces histone levels and deregulates gene expression
Chronic γ-radiation increases nuclear size and global RNA synthesis. (a) Nuclei of fibroblasts exposed to chronic γ-radiation for 7 days, stained with DAPI. All images were acquired by fluorescent microscopy with 10X objective under similar conditions and quantified by automated software detection and nuclear area calculated for >1000 cells. (b) Global RNA synthesis in primary keratinocytes and fibroblasts; detected by pulse labelling with Alexa 488-labelled EU and ensuing measurement of average fluorescence intensity per cell. *Is p < 0.1, *** is p < 0.001 relative to un-irradiated samples.
