Table 1 List of antibodies used.
From: Chronic irradiation of human cells reduces histone levels and deregulates gene expression
Supplier | Cat. No. | Application | Dilution | |
|---|---|---|---|---|
53BP1 | Millipore | MAB3802 | IF | 1:200 |
ATM | Abcam | ab32420 | WB | 1:5000 |
ATM-P (S1981) | Abcam | ab81292 | WB | 1:5000 |
Chk1-P (S345) | Cell Signalling | 2348 | WB | 1:1000 |
GAPDH | Santa Cruz | sc-25778 | WB | 1:10000 |
H2A.J | Active Motif | 61793 | WB | 1:1000 |
H2AX | Bethyl Lab | A300-082A | WB | 1:5000 |
γH2AX | Cell Signalling | 9718S | IF/WB | 1:200/1:5000 |
Histone H1 | Santa Cruz | sc-8030 | WB | 1:500 |
Histone H2A | Abcam | ab18255 | WB | 1:2000 |
Histone H2A.Z | Abcam | ab150402 | WB | 1:1000 |
Histone H2B | Abcam | ab1790 | WB | 1:20000 |
Histone H3 | Novus Biologicals | NB500-171 | WB | 1:20000 |
Histone H4 | Novus Biologicals | NBP2-16848 | WB | 1:10000 |
Lamin B1 | Abcam | ab16048 | WB | 1:5000 |
p21 | Abcam | ab18209 | WB | 1:5000 |
p53 | Santa Cruz | sc-126 | WB | 1:10000 |
p53-P (S6) | Cell Signalling | 9285 | WB | 1:1000 |
p53-P (S15) | Cell Signalling | 9284 | WB | 1:1000 |
p53-P (S46) | Cell Signalling | 2521 | WB | 1:1000 |
