Figure 4
From: Post-mortem Plasma Cell-Free DNA Sequencing: Proof-of-Concept Study for the “Liquid Autopsy”
Sanger sequencing of the DNA extracted from formalin-fixed paraffin-embedded metastases. (a) Systemic distribution of clones harboring representative cell-free DNA (cfDNA)-specific mutations (N = 8) and mutations commonly detected in eight frozen metastases (N = 3). WT, wild type; NA, data not available due to unsuccessful sequencing. This figure is drawn using Excel 2016 and PowerPoint 2016, Microsoft Cooperation, Redmond, WA, USA. (b) One of the cfDNA-specific mutations, PRKAR1A (c. 996T > G), was identified in one of the liver metastases (Liver 10). Other metastatic nodules, even those that were in close proximity (Liver 11, 12, and 13), harbored wild-type PRKAR1A. Another cfDNA-specific mutation, BCL11B (c. 391G > A), was identified in one of the paraaortic lymph node metastases.
