Figure 1

UPAR expression in bladder and breast cancer cell lines. Bladder and breast cancer cell lines resembling respectively different grades (RT4 -grade 1-, RT112 and 5637- grade 2-, HT1376 and ECV304 -grade 3-) and subtypes (MDA-MB-468, BT549, SUM149, SUM159 -TNBC-, SKBR3 -HER2⁺-) were analyzed by quantitative PCR (see “Materials and Methods”) for UPAR gene expression (A) and by flow cytometry for uPAR protein expression (b and c). UPAR protein expression is shown as histogram plots (B) and Relative Fluorescence Intensity (RFI) (see “Materials and Methods”) (C). SKBR3 HER2⁺ breast cancer cell line was used as subtype control. The dashed line represents the threshold arbitrarily defining positive expression (RFI = 2).