Figure 2
From: A novel de novo variant of GABRA1 causes increased sensitivity for GABA in vitro
Protein expression of α1 in HEK293 cells. (a) Expression of total α1 protein was detected in HEK293 cells transfected with wild-type or mutant human α1β3γ2 cDNAs (ratio 1:1:5). Protein levels were normalised to GAPDH expression. (b) Representative western blot analysis (n = 3). 1: 20 µg mouse Hippocampus, 2: 20 µl untransfected HEK293 cells, 3–5: 20 µl HEK293 α1A332Vβ3γ2, 6–8: 20 µl HEK293 α1β3γ2; bg1: background signal for green channel (α1), bg2: background signal for red channel (GAPDH). (c) Immunostaining of surface α1 expression (red). Both mutated and wild-type subunits appear at cell surfaces when co-expressed with β3 and γ2 subunits.
