Figure 3
From: A novel de novo variant of GABRA1 causes increased sensitivity for GABA in vitro
3H-flunitrazepam displacement in transfected HEK293 cells. 3H-flunitrazepam displacement with increasing concentrations of diazepam was performed in HEK293 cells transfected with human α1β3γ2 cDNA with either wild-type α1 or mutant α1A332V. Values depict mean ± SEM from six individual experiments. IC50 values for α1β3γ2 and for α1A332Vβ3γ2 are 33 nM and 23 nM, respectively. Statistically significant differences were determined by two-tailed students t-test, where p < 0.05; **p < 0.01.
