Figure 4
From: A novel de novo variant of GABRA1 causes increased sensitivity for GABA in vitro
GABA dose response relationships in wild-type and mutated receptors. (a,b) GABA dose response curves of all four wild-type and mutated receptors. Data were fitted to a sigmoidal dose response with a variable slope and points are depicted as mean ± SEM. Each data point represents experiments from n = 7–12 oocytes from ≥2 batches. (c,d) The column graphs depict the pEC50 values of α1A332Vβ3, α1β3, α1A332Vβ3γ2 and α1β3γ2 receptors obtained by fitting data of each cell individually. Statistically significant differences were determined by two-tailed students t-test, where p < 0.05; ****p < 0.0001. (e,f) Representative traces from electrophysiological recordings in α1β3 (e) and α1A332Vβ3 (f) receptors. (g,h) Representative traces from electrophysiological recordings in α1β3γ2 (g) and α1A332Vβ3γ2 (h) receptors.
