Figure 5
From: A novel de novo variant of GABRA1 causes increased sensitivity for GABA in vitro
(a) Est. PO(max) values of α1β3γ2 and α1A332Vβ3γ2 receptors. The values were determined by dividing the current elicited by 3 mM GABA by the current elicited by 10 mM GABA, 1 µM diazepam, and 3 µM etomidate and corrected for the reference 3 mM GABA current. Lines and error bars represent the mean ± SEM of 4 individual cells. (b) Representative traces from electrophysiological recordings in α1β3γ2 and α1A332Vβ3γ2 receptors after application of reference 3 mM GABA and 10 mM GABA, 1 µM diazepam, and 3 µM etomidate, respectively. (c) Est. PO(max) values of α1β3 and α1A332Vβ3 receptors. The values were determined by dividing the current elicited by 3 mM GABA by the current elicited by 10 mM GABA, 10 µM LAU 176, and 3 µM etomidate and corrected for the reference 3 mM GABA current. Lines and error bars represent the mean ± SEM of 4–6 individual cells. (d) Representative traces from electrophysiological recordings in α1β3 and α1A332Vβ3 receptors after application of reference 3 mM GABA and 10 mM GABA, 10 µM LAU 176, and 3 µM etomidate, respectively. Statistically significant differences were determined by two-tailed students t-test, where p < 0.05; **p < 0.01.
