Figure 2
From: TAK1 signaling regulates p53 through a mechanism involving ribosomal stress
Assessment of the TAK1 signaling network in the regulation of p53. (A-C) Immunoblots of whole-cell lysates from MCF7, A549 and WI-38 cell lines treated for indicated time with TAK1-i 5 µM 5Z-7-oxozeaenol (Oxo) or 10 µM CAY10657 (CAY), IKKα/β inhibitor 10 µM BMS-345541 (BMS), MEK inhibitor 5 µM U0126 (U126), or Smac-mimetic cIAP inhibitor 0.1 µM TL-32711 (SM). (D) Human colon cancer HCT-116 and isogenic HCT-116-p53ko cell lines were treated for indicated time with TAK1-i 5 µM 5Z-7-oxozeaenol (Oxo) or IKKα/β inhibitor 10 µM BMS-345541 (BMS). Graphs show fold changes relative to control in the GAPDH-normalized band density.
