Figure 8
Replication assay of i-motif containing sequence having (A) VEGF IM without Fis, (B) VEGF IM with 100 µM Fis, (C) c-MYC IM without Fis, and (D) c-MYC IM with 100 µM Fis. (E) QSTR plot of −∆G°37 values vs. lnks for reactions to dissolve the stall from reaction start along VEGF IM with or without Fis. The linear lines indicate i-motif–forming templates (blue) and hairpin forming template (green). All the replication assays were carried out in 40 mM MES (pH 6.0), 50 mM KCl, 8 mM MgCl2, 1 μM KF exo-, 1 μM DNAs, and 250 μM dNTPs at 37 °C.
