Figure 3

NS8593 blocks TRPM7 currents in kidney cells. (a) Inhibitory effects of NS8593 on TRPM7 current in NRK-52E cells was investigated using whole-cell patch-clamp recordings. Average TRPM7-mediated outward currents at +80 mV and inward currents at −80 mV extracted from ramp currents delivered at 0.5 Hz and plotted as a function of time. 10 μM NS8593 (black circle, n = 15) was applied from 150 s (black bar). 0 μM NS8593 contained DMSO vehicle and was used as control (white circle, n = 8). (b) Average I/V curve of TRPM7 currents extracted before (148 s, dot lines) and after (300 s, solid lines) NS8593 application. Black lines represent control cells and red lines are NS8593 treatment cells. (c) Same experimental protocol as in (a), but recorded in NRK-49F cells. Black circles represent 10 μM NS8593 application (n = 11) and white circles represent vehicle control (n = 6). (d) Average I/V curve of TRPM7 on NRK-49F as in (b).