Figure 5
NS8593 suppresses TRPM7 expression in renal damage. (a) The level of TRPM7 mRNA expression was examined using UUO kidneys with (black bars) or without NS8593 treatment (white bars). *p < 0.005 compared to CLK kidneys in the non-treatment control group. (b) Representative slides of TRPM7 immunostaining (magnification x200) in the non-treatment (upper panels) and NS8593 treatment group (lower panels) both from CLK (left panels) and UUO kidneys (right panels). Scale bars are 100 μm. Triangular arrows indicate epithelial cells with TRPM7-high expression (black), TRPM7-low expression (white) and non-detectable (blue). Arrows point to interstitial cells that are either TRPM7-positive (black) or TRPM7-negative (white). (c) The graph plots the analysis of TRPM7 expression in tubular epithelial cells assessed in 10 representative and non-overlapping slides (negative, white bars; low expression, grey bars, high expression, black bars). The average tubular epithelial cell number per field was 338 cells in non-treatment CLK kidneys, 359 cells in CLK kidneys with NS8593 treatment, 418 cells in non-treatment UUO kidneys and 396 cells in UUO kidneys with NS8593 treatment. *p < 0.05 vs. CLK kidney with NS8593 treatment, **p < 0.005, ***p < 0.001 vs. CLK kidney with non-treatment, #p < 0.005, ##p < 0.001; UUO kidneys with non-treatment vs. NS8593 treatment. (d) Percentage of TRPM7-positive interstitial cells assessed in non-treatment control kidneys (white bars) versus NS8593 treatment (black bars). The average interstitial cell number per field was 90 cells in non-treatment CLK kidneys, 104 cells in CLK kidneys with NS8593 treatment, 207 cells in non-treatment UUO kidneys and 142 cells in UUO kidneys with NS8593 treatment. *p < 0.05 vs. CLK kidney with non-treatment, **p < 0.005 vs. CLK kidney with NS8593 treatment, ***p < 0.001 vs. CLK kidney with non-treatment, #p < 0.005 comparing UUO kidneys in non-treatment vs. NS8593 treatment groups.
