Table 1 SNPs targeted in the ASO-PCR designed to survey the Callao-1 strain.
Targeted SNP | Primer sequences | PCR product size (bp) | DNA target |
|---|---|---|---|
A 2365238 G | IG2135-F:5′-CCTTGGCATCTTTGCGATCC-3′ | 350 | All strains except prison present branch variants |
IG2135-Rwt: 5′-GGCAGGCGCAAACGTT-3′ | |||
G 618771 T | Rv0528-F: 5′-GCTGTTCGTGTCCCTCGT-3′ | 271 | Non–Callao-1 |
Rv0528-Rwt:5′-GAAGTGGAACACCAGGTTGC-3′ | |||
G 3411528 A | Rv3050c-F: 5′-GTCAGATGCGCCACGAAC-3′ | 218 | Callao-1 (all variants) |
Rv3050c-Rmut: 5′-GCGACGGTACGCACCT-3′ | |||
A 4238842 G | Rv3792-Fmut: 5′-AATCAAGGCGGCGGTAGG-3′ | 155 | Callao-1 (all variants) |
Rv3792-R: 5′-CGTCTGCGGGTAGGTAGTG-3′ |
