Figure 13 | Scientific Reports

Figure 13

From: Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2

Figure 13

Administration of pyrocatechol significantly prevents the LPS-induced expression of CCL2, CXCL1, IL-6, and TNFα in mice. Male C57BL/6 mice were given water or 74.4 μM of pyrocatechol, which is equivalent concentration to the content of pyrocatechol in 60% (v/v) coffee, for 3 weeks and then injected intraperitoneally with PBS or LPS (200 μg/mouse) (6 mice per group). Seven hours after the injection of PBS or LPS, total RNA was extracted from the lungs (A), liver (B), and kidneys (C). The mRNA expression of CCL2, CXCL1, and IL-6 was assessed by RT-PCR. GAPDH mRNA was analyzed as an internal control. Values are means ± SD from six independent experiments. *, **, and ***indicate p < 0.05, p < 0.01, and p < 0.001, respectively.

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