Figure 6 | Scientific Reports

Figure 6

From: Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2

Figure 6

Extract of roasted coffee beans induces the expression of Nrf2, which negatively inhibits LPS-induced inflammatory responses. Transfected RAW264.7 cells with control siRNA (si-control) or siRNA against Nrf2 (si-Nrf2) were pretreated with coffee extract (5% (v/v)) for 1 h prior to the stimulation with LPS (1 μg/mL) for the indicated periods. (A) Nitrate concentrations in culture supernatants were measured 24 h after the LPS stimulation using Griess reagent. (B) iNOS mRNA expression was evaluated 12 h after the LPS stimulation by RT-PCR. GAPDH mRNA expression was used as an internal control. (C) The amounts of CCL2, CXCL1, IL-6, and TNFα in supernatants were evaluated 24 h after the LPS stimulation by ELISA. (D) The mRNA expression of CCL2, CXCL1, IL-6, and TNFα was assessed 2 h after the LPS stimulation by RT-PCR. The expression of GAPDH mRNA was used as an internal control.

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