Figure 5
Lung surfactant decreases ASMA expression in wounds on day 14 (a,b) Alph-smooth-muscle actin (ASMA) analysis by gene array. An increase of ASMA expression was found in all groups compared to control on d8 (a). Significantly reduced ASMA was found with both Alv treatments on d14 (b, see also Fig. 2). (c,d) ASMA positivity in wounds (arrows) was assessed by scoring 0–3 of immunohistochemical sections. Wound margins and centres had a trend to less ASMA with Alv compared to controls on d14 (c,d) with significantly less ASMA in FG (*p = 0.048). (e) epidermis, d dermis, g granulation tissue, FG fatty gauze. Scale bar in d14 for all sections: 200 µm. (d) White bars 0 control, green bars 0.01 Alv, blue bars 0.5 mg/ml Alv, black bars fatty gauze. d8: n = 12 all groups, d14: n = 12 control, Alv 0.5, n = 10 Alv 0.01, n = 8 FG. Mean ± SEM.
