Figure 1
From: Cerebrospinal fluid proteomics implicates the granin family in Parkinson’s disease
Large-scale mass spectrometry-based biomarker discovery. (a) DIA-MS approach overview. (b–e) Library generation and data acquisition workflow. To generate the library for processing samples by DIA-MS, a representative sample subset (b) and fractionated CSF pool (c) were processed and run in DDA-mode. (d) The gene ontology (go)-term bioprocess (left) classification (pantherdb.org)20,21 of the protein library consisting of 341 quantifiable protein groups. Proteins involved in pathways implicated in PD are indicated on the right. (e) CSF samples (see Table 1) were processed and analyzed by DIA-MS. A representative total ion chromatogram is shown on right. SPE = solid phase extraction, DDA = data-dependent acquisition.
