Figure 2
From: The scaffold protein IQGAP1 is crucial for extravasation and metastasis
IQGAP1 knockout in MA2 cells reduces experimental metastasis in vivo. (A) Western blot of IQGAP1 and GAPDH in parental (WT), clonal IQGAP1 knockout (sg) and rescue (+IQGAP1) MA2 lines. Molecular weight markers in kDa are indicated. Full-length IQGAP1 is shown with an arrow. Blot is cropped for clarity, with full-length blot presented in Supplementary Fig. S7B. (B–D) Tail-vein injection of clonal IQGAP1 knockout and rescue lines, each injected individually. n = 10–19 mice per group. (B,C) Number of metastases observed, with all clonal knockout and rescue lines shown pooled (B) or separated by clonal line (C). (D) Representative images of lungs 6 weeks after injection. Scale bar, 10 mm. *P ≤ 0.05; **P ≤ 0.01; one-way ANOVA with Tukey’s multiple comparisons test.
