Figure 5
From: The scaffold protein IQGAP1 is crucial for extravasation and metastasis
Effects of mutant forms of IQGAP1 on experimental metastasis in MA2 cells. (A) Domain structure of human IQGAP1, including calponin homology (CH), coiled coil (CC), WW, IQ, GAP-related domain (GRD) and RasGAP C-terminal (RGCT) domains, adapted from Brown and Sacks27. Shown below are regions of IQGAP1 deleted or mutated in the IQGAP1 mutant constructs used in this study: IQGAP1ΔIQ, IQGAP1-T1050AX2 (IQGAP1-T), IQGAP1-S1441A/S1443A (IQGAP1-AA) and IQGAPΔCT. (B,C) Tail-vein injection of parental (WT) or clonal IQGAP1 knockout line sg2a, rescued with either WT or mutant IQGAP1. n = 5–11 mice per group. (B) Fraction of lung surface area occupied by tumors. (C) Representative images of lungs 6 weeks after injection. Scale bar, 10 mm. ns, *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; one-way ANOVA with Šidák’s multiple comparisons test.
