Figure 6
From: Inhibition of bone morphogenetic protein 6 receptors ameliorates Sjögren’s syndrome in mice
Expression of aquaporin 5 in submandibular glands of C57BL/6.NOD-Aec1Aec2 mice. Confocal images of submandibular gland (SMG) tissue of LDN-treated C57BL/6.NOD-Aec1Aec2 mice. (A) Representative Z-stack images from SMG slides labeled with isotype control antibody anti-mouse AQP5 antibody for mice treated with PBS (left column), LDN-212854 (middle column) or LDN-193189 (right column). Row 1: slides labeled with AQP5 antibody (40× objective, white bar: 20 µm). Row 2: Inset for the marked area in row 1 (red-dashed box) (N ≥ 3). (B) Statistical analysis of AQP5 expression measured by total volume from Z-stack images in panel A showed a significant increase in AQP5 expression in LDN-treated mice compared with control mice (P < 0.0001). Data shown are means ± SEM. Statistical significance was determined with unpaired Student’s t test.
