Figure 5 | Scientific Reports

Figure 5

From: Integrative proteomic and functional analyses provide novel insights into the action of the repurposed drug candidate nitroxoline in AsPC-1 cells

Figure 5

Nitroxoline promotes intracellular ROS production and Ser139H2AX phosphorylation. (A) Intracellular ROS levels in AsPC-1 cells were measured by flow cytometry using DCFH-DA after 24 and 48 hours of nitroxoline treatment. Data shown are the means ± SD of three independent experiments (**p < 0.01; ***p < 0.001). (B) Western blot analysis of pSer139H2AX at both 24 and 48 hours of nitroxoline treatment. Full-length of these representative western blots are showed in Supplementary Fig. S4.

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