Figure 1

Identification of JHSB3 from the hemolymph of R. prolixus. (A) Structure of JHSB3. Epoxide groups are in red. The methyl esters group is in blue. (B) Typical ion extracted chromatograms of the four transitions (e.g., 283 → 233, 283 → 145, 283 → 119, and 283 → 205) utilized to verify the presence of JHSB3 in the hemolymph (left) and a JHSB3 standard (right). (C) Typical ultra-high, resolution FT-ICR MS/MS spectrum of a JHSB3 standard. The parent and the four fragment ions utilized for the experimental JHSB3 verification are labeled in the spectra.