Figure 2

JHSB3 titer in the hemolymph of 4th instar of R. prolixus. Nymphs were blood-fed and hemolymph was collected at different days after blood feeding until molting to 5th instar (10 days later). Day 0 represents insects just before feeding. Each data point represents the mean ± SEM of at least four independent replicates of hemolymph of groups of 15 nymphs each.