Figure 1
From: RNA sequence and length contribute to RNA-induced conformational change of TLS/FUS
The hypothesized closed-to-open conformational change of TLS on binding to pncRNA for the repression of CCND1 and the strategy for the detection of the conformational change by FRET. (a), The mechanism of the repression of CCND1 by TLS on DNA damage7. A closed-to-open conformational change of TLS is hypothesized on binding pncRNA, which enables TLS to interact with CBP/p300 and repress their histone acetyltransferase activities, resulting in the repression of CCND1. (b), Schematic illustration of the protein used in this study, MBP-BFP-TLS-GFP-6xHis. Residue numbers of TLS are indicated. Positions of ALS-linked mutations, K510R and P525L, are also indicated. (c), The strategy for the detection of the closed-to-open conformational change of TLS by FRET. (d), 10% SDS-PAGE of MBP-BFP-TLS-GFP-6xHis, arrowhead (right), and the molecular mass markers (left). These are cropped gels. The uncropped full-length gel is presented in Supplementary Fig. S2 online.
