Figure 2
From: RNA sequence and length contribute to RNA-induced conformational change of TLS/FUS
Detection of the closed-to-open conformational change of TLS on binding of pncRNA by FRET. (a), The localization, length, sequence and secondary structure of pncRNA21. (b), Fluorescence spectrum of TLS (100 nM), excited at 402 nm, in either the absence (blue) or presence (red) of an equimolar amount of full-length pncRNA of 602 nt. (c), The names and sequences of fragments of R31 of pncRNA. (d), Fluorescence spectrum of TLS in either the absence (blue) or presence (red) of an equimolar amount of R31 of pncRNA. (e), Absolute values of the change in the relative FRET efficiency, ∆E, where E = IGFP/ (IGFP + IBFP), on the addition of each fragment of R31 of pncRNA. Three independent results were averaged for each fragment. |∆E| is presented as a mean ± standard deviation. *indicates p value < 0.05. (f), |∆ E| of R6, GAGGGU, and its mutants.
