Table 3 Putative restriction modification (RM) systems and target motifs found in N. meningitidis sublineage 1 (n = 5) and sublineage 2 (n = 3) isolates.
Motif | Detected by | Fraction (%) | RMS Type | Meth type | REBASE entry | Corresponds to NEIS locus | Product | ORF status (frequency) | |
|---|---|---|---|---|---|---|---|---|---|
Sublineage 1 | Sublineage 2 | ||||||||
CACNNNNNTAC | PacBio | 93–99 | I gamma | m6A | M.Nme12ORF4850P | NEIS2535 | Type I restriction enzyme system specificity protein | OFF (5/5)d | ON (3/3) |
– | REBASE | I gamma | m6A | M.Nme12ORF2755P | NEIS2361 | NgoAV Type I RM system, DNA methyltransferase subunit M | ON (5/5) | ON (3/3) | |
GATC | PacBio | 94–100 | II alpha | m6A | M1.Nme12ORF55P | NEIS0327 (dam) | DNA adenine methylase | ON (5/5) | ON (3/3) |
GATC | REBASE | II beta | m6A | M2.Nme12ORF55P | NEIS0328 (dpnIIB) | modification methylase | ON (4/5) | ON (3/3) | |
– | REBASE | II gamma | m6A | Nme12ORF6960AP | NEIS2524 | hypothetical protein | ON (5/5) | ON (3/3) | |
– | REBASE | II gamma | m6A | Nme12ORF6960BP | NEIS2523 | putative methyltransferase | ON (5/5) | ON (3/3) | |
CCWGGa | REBASE | II | m5C | M.Nme12ORF5195P | NEIS2442 | DNA cytosine methylase | ONe (5/5) | ONe (3/3) | |
GGNNCCa,b | PacBio | 75–100 | II | m5C | M.Nme12ORF4970P | NEIS1180 (nlaIV) | DNA modification methylase | ON (5/5) | ON (3/3) |
– | REBASE | II | m5C | M.Nme12ORF3425P | NEIS2555 | DNA cytosine methylase | ON (5/5) | ON (3/3) | |
CCAGAa | REBASE | II | m5C | M.Nme12ORF2740P | NEIS0771 | DNA cytosine methylase | ON (5/5) | ON (3/3) | |
GGTGAc | REBASE | II | m6A | M1.Nme12ORF10280P | NEIS2910 | modification methylase | OFFf (5/5) | OFFf (3/3) | |
– | REBASE | II | m5C | M2.Nme12ORF10280P | NEIS2854 | D12 class adenine-specific DNA methyltransferase | ON (5/5) | ON (3/3) | |
ACACCb | PacBio | 70 | III beta | m6A | M.Nme12IA | NEIS1310 (modA) | Type III RM system methyltransferase (ModA12) | OFF (5/5) | OFF (2/3) |
